Journal: Metabolism: clinical and experimental

Article Title: Effects of Equivalent Sympathetic Activation during Hypoglycemia on Endothelial Function and Pro-Atherothrombotic Balance in Healthy Individuals and Obese Standard Treated Type 2 Diabetes

doi: 10.1016/j.metabol.2016.09.001

Figure Lengend Snippet: Baseline (BSL) levels of pro-inflammatory, pro-atherothrombotic and pro-coagulation markers in overnight fasted healthy and type 2 diabetic individuals during hyperinsulinemic Day 1 Euglycemia and Day 2 Hypoglycemia

Article Snippet: Vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), E-selectin, tumor necrosis factor-alpha (TNF-α) and vascular endothelial growth factor (VEGF) were assayed using LINCO Research Kits (St. Charles, Missouri) with an interassay CV of 8.5%, 9.7%, 13.4%, 9.98% and 10% respectively.

Techniques:

Journal: Metabolism: clinical and experimental

Article Title: Effects of Equivalent Sympathetic Activation during Hypoglycemia on Endothelial Function and Pro-Atherothrombotic Balance in Healthy Individuals and Obese Standard Treated Type 2 Diabetes

doi: 10.1016/j.metabol.2016.09.001

Figure Lengend Snippet: Effects of hyperinsulinemic euglycemia (5 mmol/L) and hyperinsulinemic hypoglycemia (3.2 mmol/L and 2.9 mmol/L) on VEGF, Endothelin-1 and FMD. Responses from baseline to end of clamps in overnight fasted type 2 diabetes and healthy individuals. Values are mean±SE. *p<0.01–0.0001 significantly different from baseline, † p<0.05–0.004 significantly different from healthy individuals, ‡ p<0.02–0.004 significantly different from euglycemia, § p=0.05 significantly different from baseline.

Article Snippet: Vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), E-selectin, tumor necrosis factor-alpha (TNF-α) and vascular endothelial growth factor (VEGF) were assayed using LINCO Research Kits (St. Charles, Missouri) with an interassay CV of 8.5%, 9.7%, 13.4%, 9.98% and 10% respectively.

Techniques:

Journal: Annals of Translational Medicine

Article Title: Notoginsenoside R2 induces colonic microvascular injuries via regulating the Rap1GAP/PI3K/Akt signaling pathway

doi: 10.21037/atm-21-5898

Figure Lengend Snippet: NGR2 increased hypoxia in the colon mucosa with an increased ratio of serum VEGFA165/VEGFA121 and increased protein expression of Rap1GAP and TSP1. (A) The protein expression levels of VEGFR2, VEGFR2 (p1175), VEGFR2 (p1214), Rap1GAP, TSP1, and HIF-1α in the colon mucosa; (B,C) the serum contents of VEGFA165 and VEGFA121 and the ratio of VEGFA165/VEGFA121; (D,E) the concentration of intracellular pyruvate and lactic acid in the colonic mucosa. The data are means ± SEM of 3 independent experiments performed in triplicate. One-way ANOVA and Student’s t-test (**, P<0.001 vs. control).

Article Snippet: VEGFA121 (no: SEB851Ra) and VEGFA (no: SEA143Ra) ELISA kits were purchased from Cloud-Clone Co. Anti-VEGFR2 antibody (ab11939), anti-VEGFR2 (phospho Y1214) antibody (ab5475), and anti-Rap1GAP antibody (ab32373) were purchased from Abcam Co. (London, United Kingdom).

Techniques: Expressing, Concentration Assay, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: Fibroblast Growth Factor 2 Promotes Bladder Hypertrophy Caused by Partial Bladder Outlet Obstruction

doi: 10.3389/fcell.2021.630228

Figure Lengend Snippet: Fgf2 expression in the development of PBOO and correlation with clinical data. Venny plot (A) show that Fgf2 and Gas6 were common among high expression in week 4, low expression in week 5 PBOO rat and characteristic for secreted protein. Fgf2 expressed in free-disease bladder tissues (B) . Fgf2 expressed in different cell types of bladder tissues (C) . The level of Fgf2 in rat serum ( n = 3, female) from 0∼6 weeks (D) . Western blot show Fgf2 expression in normal, week 4 and week 5 PBOO rat bladder tissues (E) . Fgf2 promote smooth muscle cell and fibroblasts proliferation in CCK8 assay (F) . Heatmap plot show 7 low and 10 high expression gene were distributed between PBOO and normal bladder tissues (G) . GSEA analysis show three major pathway changes in PBOO rat bladder tissues compared normal (H–J) . BPH patients exhibited higher expression levels of FGF2 (K) and BDNF (O) in the serum. Pearson correlation between the level of FGF2 in serum of BPH patients and prostate volume (L) , age (M) and BMI (N) . Pearson correlation between the level of BDNF in serum of BPH patients and prostate volume (P) , age (Q) , and BMI (R) . *** p < 0.001.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for rat serum BDNF (SEA011Hu and SEA011Ra) and fibroblast growth factor 2 (FGF2) (SEA551Hu and SEA551Ra) antigen detection were purchased from CLOUD-CLONE Co. (Wuhan, China).

Techniques: Expressing, Western Blot, CCK-8 Assay